Shadrack Kiwanga

As laboratory scientist in team of researchers on Molecular Surveillance of Malaria in mainland Tanzania (MSMT) at IHI. i posses the following responsibilities.

• preparing Dried Blood Spots (DBS) from patients blood collected at field.
• Doing pre-processing of DBS containing samples, which include sorting and punching.
• Extracting DNA from punched spots bu using chelex-100
• Performing qPCR for malaria parasites' species identification, parasitemia and tracking hrp2/3 gene deletion.
• Using Illumina platform (MiSeq and NextSeq) to perform targeted sequencing of malaria parasites DNA by using MIPs and MAD4HatTeR protocols. (monitoring Drug resistance, population diversity, diagnostics and Differentiation).
• Performing Whole Genome Sequencing with Selective Whole Genome Amplification (WGS/sWGA) of malaria parasites' genome using Oxford Nanopore Technologies (ONT).
• Performing Bioinformatics analysis of generated data.

• Prepared biological samples particularly DNA for analysis.
• Operated laboratory equipment for DNA extraction and analysis.
• Analyzed raw data generated from various analytical techniques like Convetional-PCR, RT-PCR and real time-PCR
• Maintained accurate records of laboratory procedures.
• Collaborated with team members on research projects and data collection.
• Followed safety protocols to ensure a secure working environment.

• Conducted routine tests on samples to ensure accuracy and reliability.
• Maintained laboratory equipment through regular calibration and troubleshooting procedures.
• Assisted in preparing chemical solutions for various diognosis.
• Documented test results and maintained detailed records for compliance purposes.
• Followed safety protocols to maintain a clean and organized laboratory environment.
• Collected, labeled and stored lab samples according to laboratory specifications.
• Completed lab tests and communicated results to supervising staff.